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KMID : 0604520180440030349
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Journal of the Society of Cosmetic Scientists of Korea
2018 Volume.44 No. 3 p.349 ~ p.362
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Antioxidant, Antiaging and Antimicrobial Effects of Ethanolic Extract and Ethyl Acetate Fraction from Eclipta prostrata
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Lee Sang-Rae
Song Ba-Reum
Shin Hyuk-Soo
Lee Yun-Ju
Park Soo-Nam
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Abstract
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An annual plant, Eclipta prostrata (Linn) is a member of the Asteraceae plant family and inhabited in tropical or subtropical regions of the world. Through many previous researches, E. prostrata has been extensively studied for its hepatoprotective effect, antivenom potential against viper venom, antioxidant, hair-growth, wound-healing efficacy and so on. In this study, for better understanding of the potential of E. prostrata as skin protectant, we conducted the experiments evaluating the antioxidant and antiaging efficacy. To this end, 50% ethanolic extract of E. prostrata and its ethyl acetate fraction were prepared and investigated. For the evaluation of antioxidant capacity of the samples, FSC50 and OSC50 were estimated. As a result, OSC50 of ethyl acetate fraction was 2.7 times superior to OSC50 of L-ascorbic acid, a well known antioxidant agent. Futhermore E. prostrata showed notable reactive oxygen species (ROS) scavenging coueffect and protective effect against H2O2 in the celluar level as well. Especially, in the 1O2 induced hemolysis test, 64 μ g/mL of ethyl acetate fraction showed greater than 6 times increased retardation effect compare to control which means E. prostrata has remarkable antioxidant capacity. To validate the antiaging effect of the samples, we conducted elastase inhibition assay using elastase solution extracted from human skin fibroblasts, Hs68. As a result, 16 μ g/mL of each sample showed 6.8% and 14.0% of elastase inhibition respectively. Finally, antimicrobial activity of E. prostrata was assessed to validate the possibility as alternative preservative. From the result, ethyl acetate fraction showed oustanding antimicrobial activity as of methyl paraben, a well known chemical preservative. In conclusion, these results suggest that E. prostrata can be used as natural skin protectant or preservative as natural ingredient in food or cosmetics industry.
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KEYWORD
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Eclipta prostrata , reactive oxygen species , antioxidant , antiaging , antimicrobial
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